🔬 Professional Calcium Imaging Analysis

MPS is a comprehensive pipeline for processing miniscope calcium imaging data. From raw videos to publication-ready traces, MPS guides you through every step with an intuitive GUI and powerful algorithms.

Built by neuroscientists for neuroscientists, MPS implements CNMF with modern initialization, watershed-based detection, multi-penalty LASSO spatial updates, and optimized temporal updates—scaling to large datasets.

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Complete Pipeline

Motion correction, segmentation, temporal extraction, QC, and export.

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Optimized Performance

Dask-powered parallelism and memory-aware chunking.

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Real-time Visualization

Live previews and detailed terminal logs.

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Flexible Export

Zarr, NumPy, JSON, and Pickle for downstream analysis.

Download

Signed macOS app and Windows installer

⬇macOS (ZIP) ⬇macOS (DMG) ⬇Windows (EXE)

macOS 12+ • Windows 10/11 • First run sets up the environment and shows logs in Terminal/Console.

Processing Pipeline (at a glance)

Raw Videos → Deglow + Denoise → Motion Correction → 
NNDSVD Initialization → Watershed Segmentation → Spatial Merging → 
Temporal Extraction → Noise Estimation → Temporal Update (CNMF) → 
Spatial Update (LASSO) → Temporal Update (CNMF) → QC → Export
      